rabbit monoclonal anti perilipin  (Cell Signaling Technology Inc)

Journal: JBMR Plus

Article Title: IGF-1 from bone marrow Adipoq-lineage cells stimulates endocortical bone formation in mature female mice

doi: 10.1093/jbmrpl/ziag024

Figure Lengend Snippet: Quantification of serum bone markers, osteocyte factors and marrow adiposity. (A, B) Serum levels of bone formation (A) and resorption (B) markers. (C-E) Relative mRNA levels for osteocyte-derived factors as indicated. (F) Representative images for immunofluorescence staining of perilipin 1 (green) on sections of distal femur. Tissue morphology shown by differential interference contrast (DIC) imaging. (G) Quantification of adipocyte numbers in distal femur. CTRL, Igf1 f/f ; CKO, Adipoq-Cre;Igf1 f/f . Statistics: unpaired t -test, ns: non-significant, p > .05, each dot representing a single mouse.

Article Snippet: For immunostaining, sections were washed in PBS for 15 min, blocked with Antibody Diluent with BSA for 30 min at RT, and incubated with rabbit anti-perilipin monoclonal antibody (1:200, Cell Signaling Technology, #9349) overnight at 4 °C, followed by Alexa Fluor 647-conjugated F(ab’)2-goat anti-rabbit IgG (1:500, Thermo Fisher A21246) for 1 h at RT.

Techniques: Derivative Assay, Immunofluorescence, Staining, Imaging

Journal: bioRxiv

Article Title: Defensive lipid droplets are PUFA reservoirs driving bacterial clearance and inflammation

doi: 10.64898/2026.03.12.711356

Figure Lengend Snippet: (A) BV2 cells were activated for 3, 6, or 16 hours with the dMIX and Tnf , Il-6 , and Ifn β expression quantified by qRT-PCR and normalised to their expression in untreated cells (CTL). Graphs show means±SD (combined from at least N=4); *P < 0.05, **P < 0.01, ****P < 0.0001 in an ordinary one-way ANOVA. Red and blue asterisks indicate respectively significant enrichment or reduction. (B) BV2 cells were stimulated for 3, 6, or 16 hours with dMIX and “core” and “immune” LD proteins expression quantified by qRT-PCR and normalised to their expression in untreated cells (CTL). Graphs show means±SD (combined from at least N=4); ns is not significant, *P < 0.05, **P < 0.01, ****P < 0.0001 in an ordinary one-way ANOVA. Red and blue asterisks indicate respectively significant enrichment or reduction. (C) Representative confocal images (N=3) of BV2 cells treated for 16 hours with 15µg/ml OA to induce mLDs or with the dMIX to induce dLDs. Cells were labelled with anti-Igtp (green) and anti-Plin2 (red) antibodies and LipidTox (LD, blue). Scale bar is 20µm. The yellow square marks the region selected for the high magnification panels.

Article Snippet: Primary antibodies were diluted in blocking solution: mouse monoclonal anti-Viperin (1:100, ab107359, Abcam), mouse monoclonal anti-Igtp (1:100, sc-136317, Santa Cruz Biotechnology), rabbit polyclonal anti-Plin2 (1:200, 15294-1-AP, Proteintech).

Techniques: Expressing, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Defensive lipid droplets are PUFA reservoirs driving bacterial clearance and inflammation

doi: 10.64898/2026.03.12.711356

Figure Lengend Snippet: (A) Representative confocal images (N=3) of BV2 cells treated with OA (15µg/ml) to induce mLDs or dMIX (containing 15µg/ml of FAs: 40% LA, 35% OA, 23% PA, and 2% ALA). Cells were labelled with anti-Viperin (green) and anti-Plin2 (red) antibodies and LipidTox (LD, blue). Scale bar is 20µm. The yellow square marks the region selected for the high magnification panels.

Article Snippet: Primary antibodies were diluted in blocking solution: mouse monoclonal anti-Viperin (1:100, ab107359, Abcam), mouse monoclonal anti-Igtp (1:100, sc-136317, Santa Cruz Biotechnology), rabbit polyclonal anti-Plin2 (1:200, 15294-1-AP, Proteintech).

Techniques: